Reagent kit and data analysis software for smooth implementation on the Ion Gene Studio S5 Systems.
An integrated solution, providing reagent kit and data analysis software for smooth implementation on the Ion Gene Studio S5 Systems.
Key benefits of the Immunocompatibility KIT
100% in house
- More automony
- Kit for 16 reactions
- Detection of RhD & AB0
- ~ 29 h for the results
- ~ 4 hands on time
What is Immunocompatibility KIT for?
The blastocyst (D+5/D+6 embryo) is made up of cells that form the outermost layer, which are extravillous trophoblast cells. These cells are essential to help in the correct implantation of the embryo in the uterine wall and have ligands on their membrane, called leukocyte antigens (HLA-C). HLA-C ligands for KIR are divided into 2 groups, HLA-C (C1) and HLA-C (C2). Whether a person is C1 or C2 depends on the amino acid present at positions 77 and 80.
Sperm from donors or patients who carry an HLA C C2/C2, will guarantee a pregnancy in which the trophoblast cells express C2. If this genotype is associated with the maternal KIR AA there will be a greater risk of having implantation failures, recurrent miscarriages, and difficulties during pregnancy such as pre-eclampsia or premature delivery.
RhD can present 3 genotypes, +/+, +/- and -/-. It is important to detect in cases when the woman is negative and her couple positive to avoid possible complications in pregnancy. With this test is also possible to detect AB0 typing to know if they are AA, AO, BB, BO, AB or OO.
Genotyping by Immunocompatibility KIT
Cases in which the genotyping test is indicated to
The measurement of the concordance between maternal KIR receptors and paternal HLA-C will be analyzed by means of the Immunocompatibility KIT genotyping. Multiple studies have suggested the KIR-HLA-C matching with respect to pregnancy outcomes. The worst prognostic combination according to KIR-HLA-C matching is KIR AA and HLA-C2.
A D- women who carries a D+ fetus produce antibodies against antigen-D, which are generally undetected. After the first baby, in a future pregnancy these antibodies can cross the placenta, and if the fetus is D+ can induce an immunological response and cause hemolytic disease in the newborn.
All os these cases can develop implantation failures, repeat miscarriages, preeclampsia or preterm delivery.
It can be a KIT that is routinely performed for sperm and egg donors, or any couple entering an assisted reproduction cycle.
How is it performed?
From a blood sample, the DNA is extracted to be analyzed by a genetic analysis. The performance examines KIR and HLA-C of the mother and HLA-C of the father. In case of being a gametes donation, HLA-C genotype of the gamete donor (oocytes or semen) will be genotyped.