Providing reagent kit and data analysis software for smooth implementation on the Ion Gene Studio S5 Systems.

Reagent kit and data analysis software for smooth implementation on the Ion Gene Studio S5 Systems.

An integrated solution, providing reagent kit and data analysis software for smooth implementation on the Ion Gene Studio S5 Systems.

IMMUNOCOMPATIBILITY

KIT

Get in contact

Immunocompatibility KIT

ReproHealth Immunocompatibility KIT allows genetic laboratories to analyze KIR-HLA-C genotyping, RhD factor and to know your AB0 blood group.
Journey Genomics - Immunocompatibility Kit

COMING SOON!

Key benefits of the Immunocompatibility KIT

House

  • 100% in house

  • More automony

  • Less time-consuming

  • Kit for 16 reactions
  • Detection of RhD & AB0
  • ~ 29 h for the results
  • ~ 4 hands on time

What is Immunocompatibility KIT for?

In the maternal uterus, the cells that line the wall are the uterine NK (Natural Killer). These cells have membrane receptors called KIR. 15 genes and 2 pseudogenes consolidate the KIR system. 2 haplotypes make up the KIR gene content, these are A and B. Haplotype A is invariant in terms of gene content and has only one activating KIR gene: KIR2DS4. Whereas haplotype B is quite variable and has additional activating genes.

The blastocyst (D+5/D+6 embryo) is made up of cells that form the outermost layer, which are extravillous trophoblast cells. These cells are essential to help in the correct implantation of the embryo in the uterine wall and have ligands on their membrane, called leukocyte antigens (HLA-C). HLA-C ligands for KIR are divided into 2 groups, HLA-C (C1) and HLA-C (C2). Whether a person is C1 or C2 depends on the amino acid present at positions 77 and 80.

Sperm from donors or patients who carry an HLA C C2/C2, will guarantee a pregnancy in which the trophoblast cells express C2. If this genotype is associated with the maternal KIR AA there will be a greater risk of having implantation failures, recurrent miscarriages, and difficulties during pregnancy such as pre-eclampsia or premature delivery.

RhD can present 3 genotypes, +/+, +/- and -/-. It is important to detect in cases when the woman is negative and her couple positive to avoid possible complications in pregnancy. With this test is also possible to detect AB0 typing to know if they are AA, AO, BB, BO, AB or OO.

Genotyping by Immunocompatibility KIT

 KIR genotyping consists of the determination of the positive and negative KIR cluster genes, enabling the determination of the corresponding genotype. For HLA-C, the genotyping is performed by studying the SNPs that determine the corresponding substitutions in the amino acids at position 77 and 80. In addition, ABO is determined also studying the different SNPs present on the sample. Finally, the RhD determination consists on the quantification of the number of copies of this gene and also the study of SNPs that produce a weak or non-functional D allele.

Cases in which the genotyping test is indicated to

The measurement of the concordance between maternal KIR receptors and paternal HLA-C will be analyzed by means of the Immunocompatibility KIT genotyping. Multiple studies have suggested the KIR-HLA-C matching with respect to pregnancy outcomes. The worst prognostic combination according to KIR-HLA-C matching is KIR AA and HLA-C2.

A D- women who carries a D+ fetus produce antibodies against antigen-D, which are generally undetected. After the first baby, in a future pregnancy these antibodies can cross the placenta, and if the fetus is D+ can induce an immunological response and cause hemolytic disease in the newborn.

All of these cases can develop implantation failures, repeat miscarriages, preeclampsia or preterm delivery.

It can be a KIT that is routinely performed for sperm and egg donors, or any couple entering an assisted reproduction cycle.

How is it performed?

From a blood sample, the DNA is extracted to be analyzed by a genetic analysis. The performance examines KIR and HLA-C of the mother and HLA-C of the father. In case of being a gametes donation, HLA-C genotype of the gamete donor (oocytes or semen) will be genotyped.

An integrated solution, providing reagent kit and data analysis software for smooth implementation on the Ion Gene Studio S5 Systems.
Development for human genetic testing
Parque Científico y Empresarial de la UMH
Edificio Quorum III
03202 Elche – Alicante
Spain
Phone: +34 966 261 268
Information: info@journeygenomics.com
Orders: orders@journeygenomics.com